C18 30 mm * 4.6 mm (2.4 uM) columns

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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We found Thermo BDS 30 mm * 4.6 mm (2.4 uM) column is almost perfect one and validated many methods with this column. Unfortunately Thermo hid this column from web catalog ( https://www.thermofisher.com/order/catalog/product/28105-052130 ) that made us frustrating. Are there any pharmacopoeia allowed alternatives without particles size change?
Best regards,
Dmitriy A. Perlow
Check the USP. I believe (from memory) that you can make particle diameter changes so long as the column length to particle size ratio stays constant. You *do* have to pass system suitability, of course.
-- Tom Jupille
LC Resources / Separation Science Associates
+ 1 (925) 297-5374

2.4 um diameter particles do not seem to be a typical size across multiple vendors. I'd think if the pharmacopeia recommended alternatives that each would have this information available upon request (USP occasionally lists multiple stationary phases for some of their methods, at least).

Looking at this website,


Chromegabond WR C18 (ES Industries), Viva C18 and Pinnacle DB C18 (Restek), and Superspher 100 RP-18e (MilliporeSigma) may be good alternatives--none of these is available containing 2.4 um diameter particle size.

Is it possible to request a special standing order of the Thermo BDS in 2.4 uM size in 30 mm * 4.6 mm column format?
3 posts Page 1 of 1

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