Separation and quantification of two different HPMC Grades

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

8 posts Page 1 of 1
Dear all
Could you please suggest a HPLC method for separation and quantification of two HPMC grades from a tablet matrix having molecular weight difference of around 1000000.
The problem is both are co-eluting at same RT.
As I recall HPMC is a polymer and will have the same peaks, thus the RT is the same. The grade of HPMC is dependant on the quantitation of the peaks or the main peak (like PCB's). Rather than HPLC, you may be better off measuring the viscosity over time (like corn starch).
HPLC chemist wrote:
As I recall HPMC is a polymer and will have the same peaks, thus the RT is the same. The grade of HPMC is dependant on the quantitation of the peaks or the main peak (like PCB's). Rather than HPLC, you may be better off measuring the viscosity over time (like corn starch).


Hi Thanks for your response but in a compressed tablet how you can quantify by measuring viscosity as it also contain other excipients.
the concern is separation and quantification of two different grades of HPMC (having difference in molecular weight of around 1000000) from a Tablet.

I hope my concern is clear now.

Regards
Hello.
We use https://www.phenomenex.com/Products/HPLCDetail/BioSep-SEC-S/ column for water soluble polymer (chondrotine sulfate).
Best regards,
Dmitriy A. Perlow
Your concern is unfathomable to me. HPMC is an excipient and not worthy of quantitation by HPLC or any other 'expensive' instrument. While HPMC will affect validation of a process so will other variables like compression time and pressure. Thus, HPLC will not help you determine the amount or grade of HPMC (if you are trying to re-generate a drug product).

You may have to take a lot and split it to perform a PPQ (process performance qualification). There are no shortcuts!
what method u had tried for detection of hpmc on hplc
Have you tried Size exclusion chromatography?
8 posts Page 1 of 1

Who is online

In total there are 21 users online :: 0 registered, 0 hidden and 21 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 21 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry