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Negative peaks using Normal phase

Posted: Fri Jul 11, 2008 3:31 pm
by liam1978
Hi, Im getting na lot of negative peaks on my chromatography using a normal phase where I used to get positive peaks!!! This has happened before occassionally but it usually sorts itself out - is this a common occurrence with normal phase lc and why does it happen?????

Posted: Fri Jul 11, 2008 4:55 pm
by zokitano
What kind of detection method are you using? What is the composition of your mobile phase? And what type of analytes you're analyzing?