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decreasing in retention time Vs. column deterioration
Posted: Tue Jul 24, 2018 4:20 pm
by fish.vegt
Dear All
I am writing a investigation regarding to the continue sequential runs drop in retention time each time from one to the next, and I am wondering is there any deviance says there is any relationship between the retention time decrease vs. column deterioration?
Thank you,
Re: decreasing in retention time Vs. column deterioration
Posted: Tue Jul 24, 2018 6:42 pm
by HPLC chemist
As I recall the answer is no! However, there may be a requirement in the validation documentation for a RT window. If not you can always add an addendum to the AMV protocol.
Re: decreasing in retention time Vs. column deterioration
Posted: Tue Jul 24, 2018 9:59 pm
by mattmullaney
Hi, fish.vegt,
I agree with HPLC Chemist in part--the method aught to include a range of acceptable retention times per peak (or relative retention times).
Is it possible that column stationary phase deterioration may cause a reduction in retention time for peaks--yes. Is this the only cause of this particular phenomenon--no.
Feel free to refer here
http://ccc.chem.pitt.edu/wipf/Web/LCMS% ... ooting.pdf
(pgs. 5-6), and
http://www.chromatographyonline.com/ret ... case-study
Re: decreasing in retention time Vs. column deterioration
Posted: Wed Jul 25, 2018 3:49 pm
by fish.vegt
Thank you for the comment and the valuable reference