UV-linearity test failed
Posted: Fri Jul 20, 2018 4:17 pm
I performed UV-linearity test on ultimate-3000 HPLC using caffeine standers and restricted column ( coil) the test has executed many times and failed by the same results , area of caffeine 220 mg and 300 mg less than usual for 220 its 71 mAu and for 300 mg its 92 mAu , in normal cases for 220 mg 75 and for 300 102
pressure was about 1350 Psi , no technical issue in instrument UV-lamp good and everything works good .
when i replaced i coil with old one which gave a pressures about 800 Psi everything passed and looks perfect .
how the pressure effect on peak area ?!
Note: Usually i use the coil which give 1350 Psi for qualification and its passed smoothly ,but for this instrument i dont know what happen .
pressure was about 1350 Psi , no technical issue in instrument UV-lamp good and everything works good .
when i replaced i coil with old one which gave a pressures about 800 Psi everything passed and looks perfect .
how the pressure effect on peak area ?!
Note: Usually i use the coil which give 1350 Psi for qualification and its passed smoothly ,but for this instrument i dont know what happen .