Why SEC column go “Badâ€
Posted: Tue Jul 08, 2008 5:26 am
Hi
Why do SEC column go “badâ€
Why do SEC column go “badâ€
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Why SEC column go
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Posted: Tue Jul 08, 2008 8:13 am
The silica SEC columns come with a surface modification which may react. Of course the silica itself can react and thus change. Then accumulation of "dirt" can cause surface changes or plugging. I don´t see so much diffrence to columns used for adsorption chromatography.
Posted: Tue Jul 08, 2008 2:20 pm
I'm sure part of the reason lifetime may vary depends on what the actual packing material is made of. Silica packing may fail for different reasons compared to cross-linked styrene-divinyl benzene packing. I've been using a cross-linked polymer column for SEC analysis for about 3 years now, and its still kicking.
The swelling and shrinking of the cross-linked polymer packing in various solvents over time may possibly cause unwanted flow channels within the packing material. Thats my opinion, though I may be wrong.
The swelling and shrinking of the cross-linked polymer packing in various solvents over time may possibly cause unwanted flow channels within the packing material. Thats my opinion, though I may be wrong.
Posted: Tue Jul 08, 2008 4:23 pm
If you use the SEC column for the seperation of biological sample, you would find the column would quite fast get dirty . 
Column brands
Posted: Mon Jul 14, 2008 5:26 pm
Hi,
A few years ago I was using an inline carbon analyzer as one of my detectors for SEC. At first I tried to use a Waters SEC column but it was apparently bleeding carbon steadily, and creating a high and unstable baseline in the carbon detector. However, I switched to a Tosoh Biosciences TSK column and it has undetectable bleed. Some columns are more stable than others.....
Dave
A few years ago I was using an inline carbon analyzer as one of my detectors for SEC. At first I tried to use a Waters SEC column but it was apparently bleeding carbon steadily, and creating a high and unstable baseline in the carbon detector. However, I switched to a Tosoh Biosciences TSK column and it has undetectable bleed. Some columns are more stable than others.....
Dave