Chromatography Forum

Hi, I am trying to analyse a protein made up of alanine, proline and the methylated ketone.
I think as a starting point a vydac column (c18, 150 x 4.6 mm i.d. with gradient containing 0.1 % tfa in water and acetonitrile, using gradient with detection at 226 nm

Does anyone have any other ideas?


Thanks

Liv

Hi:

I have been using vydac column for more than 4 years,it works well,Iam analyzing peptide with 238DE,and I will use 214TP for large molecule analysis