Dealing with standard curve to determine concentration

[abell1234]

Hi Kankyo,

You could try including zero or force thorugh zero,but this will depend on the method. For low level evaluation using curves this is usually typical as the intercept value is 99.9% of the time a greater are value then the deg peak ,hence a negative number is obtained. Therefore y=mx ,not
y=mx+c. See what you get ,but be aware that 'including zero'and 'force through zero'are different !
hope this helps

Thanks

Andy

[AICMM]

kankyo,

Two things. 1) You should run some sort of method detection limit study wherein you determine the minimum amount of analyte necessary for some signal to noise limit (say 10:1.) 2) You should not just rely on the numbers but you should look at the chromatogram as well. Is there a peak there or not? If so, is it significantly greater than the noise level around it? Don't just trust numbers churned out by data systems.

Best regards.