Diluent Solvent

[Kaoula]

Hi all:
Please help me:

What is the effect of diluent solvent on the mechanism of separation using reverse
phase method ???

Thank you
Kaoula

[Chrom user]

Hi Kaoula,

The choice of diluent has the effect of causing analyte focusing. If the diluent used is wrong, the peak shape will not be good. You might have a broad peak or distorted peak shape.

Sometimes you can play with using water, MeOH or even the mobile phase (composition at initial condition if run gradient). Hope this help. :wink:

[Kaoula]

Hi Chrom user:

Thank you very match for your help
Please I have another question

When we change the diluent solvent the area of the tested material is change or not and this phenomena occur with electrochemical detector rather than UV detector or not ???? ............

Thank you for your advice
Kaoula

[Kostas Petritis]

Kaoula,

All the questions that you posted are mostly qualified for the "student projects" forum and not for the "liquid chromatography" one...

Briefly the answer is no the area won't change for a small diluent solvent effect, but it might if you really distort your peak due to that effect. Also if your analyte is close to its LOD you might experience "virtual" different areas even with small diluent solvent effects.

These effects are happening in your chromatographic column and are independent your detection (so yes you will see the same thing with UV or electrochemical detection).