Plant (leafs) extraction

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

18 posts Page 2 of 2
Gaetan Glauser wrote:
Hi,

The most probable reason for a plant turning from green to white (or another color) is degradation of chlorophyll due to leaf senescence. Leaves can live for quite a while in senescent conditions. In other words, it may be worth looking at which compound disappeared rather than which one appeared. Therefore, before running complex untargeted profiling by LC-MS or GC-MS, why not perform a quick quantification of chlorophyll A and B by UV spectrophotometry?


yes it is a good idea but we dont have a UV-VIS
Not even a good old simple UV/VIS spectrophotometer with cuvettes?

Anyway, chlorophyll can also be measured by LC-MS. This will confirm what your eyes (which are actually VIS detectors in this context) have already noticed: that the green color in leaves is due to chlorophyll, and that the absence of green means destruction of chloroplasts.
Gaetan Glauser wrote:
Not even a good old simple UV/VIS spectrophotometer with cuvettes?

Anyway, chlorophyll can also be measured by LC-MS. This will confirm what your eyes (which are actually VIS detectors in this context) have already noticed: that the green color in leaves is due to chlorophyll, and that the absence of green means destruction of chloroplasts.


We have plans to purchase it in the future. Also we have put in a requisition for a brand new LC-MS. Right now we have GC only. But great suggestion.
18 posts Page 2 of 2

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