Wavy baseline on agilent 1100 VWD
Posted: Mon Jun 09, 2008 11:48 pm
Column: C18 Varian
Flow 1ml/min
Gradient: First 5 minutes: 85% ACN and 15% water
next 25 minutes: 100% ACN
Everything was fine, UNTIL I switched mobile Phases from:
Water (MPA) and Acetonitrile (MPB)
To:
Water w/0.1% TFA (MPA) and Acetonitrile w/0.1% TFA (MPB)
I mixed them both well. I already replaced the lamp, that did not help.
Before I replace the pump seals, does anyone know what else I can try?
Should I flush the column with Methanol? Reverse flush the column to elute a late contaminant peak?
Thanks
Flow 1ml/min
Gradient: First 5 minutes: 85% ACN and 15% water
next 25 minutes: 100% ACN
Everything was fine, UNTIL I switched mobile Phases from:
Water (MPA) and Acetonitrile (MPB)
To:
Water w/0.1% TFA (MPA) and Acetonitrile w/0.1% TFA (MPB)
I mixed them both well. I already replaced the lamp, that did not help.
Before I replace the pump seals, does anyone know what else I can try?
Should I flush the column with Methanol? Reverse flush the column to elute a late contaminant peak?
Thanks