Isolation of poly hydroxy compounds
Posted: Fri Oct 08, 2004 8:16 am
Hi every body,
I have a problem with isolating of an unknown poly hydroxy aliphatic compound. this one has a sulfate ester. I have used ODS column and I was successful to isolate this one and after several injections i found very poor resolution and all the peaks overlaped. I have tried to use phosphate buffer (PH 6.3) but the peak still overlaped.
what do you thing? what is the reason? How can solve this problem.
I have a problem with isolating of an unknown poly hydroxy aliphatic compound. this one has a sulfate ester. I have used ODS column and I was successful to isolate this one and after several injections i found very poor resolution and all the peaks overlaped. I have tried to use phosphate buffer (PH 6.3) but the peak still overlaped.
what do you thing? what is the reason? How can solve this problem.