Chromatography Forum

Dear All,

System suitability injection made at initially there were no split peak to impurity peak but when I am injecting same system suitability injection at the end of analysis, there were split peak in impurity peak but it was not case with principal peak? Please suggest

Thanks in advance.

Narendra

Degradation as a function of time?
Try injecting this particular sample-type several times, for as long as a typical Sample Set takes to complete.
Maybe you’ll see some (further) degradation at a certain time point and thus determine the stability of this sample type.

Best Regards