Advertisement

Split peak

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
Dear All,

System suitability injection made at initially there were no split peak to impurity peak but when I am injecting same system suitability injection at the end of analysis, there were split peak in impurity peak but it was not case with principal peak? Please suggest

Thanks in advance.

Narendra

Degradation as a function of time?
Try injecting this particular sample-type several times, for as long as a typical Sample Set takes to complete.
Maybe you’ll see some (further) degradation at a certain time point and thus determine the stability of this sample type.

Best Regards
Learn Innovate and Share

Dancho Dikov
2 posts Page 1 of 1

Who is online

In total there are 31 users online :: 0 registered, 0 hidden and 31 guests (based on users active over the past 5 minutes)
Most users ever online was 11462 on Mon Dec 08, 2025 9:32 pm

Users browsing this forum: No registered users and 31 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry