Chromatography Forum

After running a sequence with set calibration levels the resulting automatically-generated calibration table is populated with incorrect peak area values. The update response factor/retention time columns are set to "replace" so it's not averaging anything with past data. If we delete the calibration table and recreate it manually, it populates with correct peak areas. Is there a setting being missed here?

Also, as an aside, is there an intelligent reporter expression that references the dilution factor for a given injection?

I don't know if this applies to your situation, but in chemstation sequences we ALWAYS had the first line (standard) as "replace" with 1 or 2 injections then the second line was same vial of standard but set as "average".

"Replace" cleared any past calibration areas.

In the method settings, you get to choose how and when to update the cal standards and when to use the recently integrated data to do so. Also, you select which "sample" consists of stds and which are actual samples. Any basic errors in these method parameters could produce the results you have observed.