TLC Separations of Lipids

[josebenjamin]

Dear friends,

I am trying to determine whether is possible to do all the following glyceride groups in a single or double TLC run. I am interested in TG, cholesterol esters, cholesterol, DG, MG, PC, PE, PI and sphingomyelin.

I would like to receive some information (PDFs, links, references, etc), and practical tips on this subject.

Thanks,

josebenjamin

[Bruce Hamilton]

The place to start for any lipid analysis information is either W.W. Christies' book "Lipid Analysis" and/or his WWW site.
http://www.lipidlibrary.co.uk/index.html

He is the acknowledged expert in the chromatographic analysis of lipids, and he's put a lot of information freely available on his site, but you should really get his book, and some of the others from Oily Press that have relevant chapters.

There is also a white 3 volume set of books ( I can't recall the authors ) on Lipid Chromatographic Analysis from the 1970s that have several chapters on TLC of lipids, and you will find that much of the research literature from the 1960s and 1970s uses TLC for lipids.

TLC is excellent for Lipids, hence the populatity of the Iatroscan ( TLC-FID ) for quantitation, but semi-quantitative visualisation is also easy
A good technical library will be a great place to start.

Please keep having fun,

Bruce Hamilton