Agilent Zorbax Columns

[harry2008]

Hi,

I should use Agilent Zorbax XDB-CN column for NP-HPLC sepn. But I couldn't find it. What I found is Agilent Zorbax SB-CN column. Can I use this column? Thanks.

harry

[Kostas Petritis]

The column you indicate is still commercially available... or do you mean that is not available in your laboratory?

[harry2008]

Thanks, Kostas. Yes, it is not available in my laboratory. I'd like to know what difference between Agilent XDB-CN and Agilent SB-CN. Can I use SB-CN column to get the same good result as I used XDB-CN column ?

[DR]

I would guess that selectivity and plate counts would be different. SB is a pretty garden-variety particle/coating configuration while XBD is not.

[tom jupille]

I believe the underlying silica is the same, but the XDB is end-capped, while the SB uses isopropyl side chains on the bonding silanes instead of the more usual methyl groups.

I just looked in the PQRI column selectivity database on the USP web site:
http://www.usp.org/USPNF/columnsDB.html (the PQRI database is the second one on the page). It contains both columns, but they are not listed among the 10 most similar each other.

[Kostas Petritis]

These column bondings (XDB vs.SB) are advertised to be providing alternative selectivities one from another so I wouldn't expect identical results. But of course both columns might provide separation between your compounds of interest...

Here are some more info about the different Agilent bonded phases:

http://www.crawfordscientific.com/DOWNLOADS/ZxSel.pdf