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Agilent Zorbax Columns
Posted: Tue May 20, 2008 6:47 pm
by harry2008
Hi,
I should use Agilent Zorbax XDB-CN column for NP-HPLC sepn. But I couldn't find it. What I found is Agilent Zorbax SB-CN column. Can I use this column? Thanks.
harry
Posted: Tue May 20, 2008 6:51 pm
by Kostas Petritis
The column you indicate is still commercially available... or do you mean that is not available in your laboratory?
Posted: Tue May 20, 2008 7:45 pm
by harry2008
Thanks, Kostas. Yes, it is not available in my laboratory. I'd like to know what difference between Agilent XDB-CN and Agilent SB-CN. Can I use SB-CN column to get the same good result as I used XDB-CN column
?
Posted: Tue May 20, 2008 8:08 pm
by DR
I would guess that selectivity and plate counts would be different. SB is a pretty garden-variety particle/coating configuration while XBD is not.
Posted: Tue May 20, 2008 10:58 pm
by tom jupille
I believe the underlying silica is the same, but the XDB is end-capped, while the SB uses isopropyl side chains on the bonding silanes instead of the more usual methyl groups.
I just looked in the PQRI column selectivity database on the USP web site:
http://www.usp.org/USPNF/columnsDB.html (the PQRI database is the second one on the page). It contains both columns, but they are not listed among the 10 most similar each other.
Posted: Wed May 21, 2008 5:41 am
by Kostas Petritis
These column bondings (XDB vs.SB) are advertised to be providing alternative selectivities one from another so I wouldn't expect identical results. But of course both columns might provide separation between your compounds of interest...
Here are some more info about the different Agilent bonded phases:
http://www.crawfordscientific.com/DOWNLOADS/ZxSel.pdf