Seperation of PEG for GC analysis

[mimeo]

Hi there,

Can anyone tell me how to derivitize and seperate PEG 400 for GC analysis? Does the GC oven temperature have to be a high temp one?

Thanks in advance for any help or advice!


Rebecca

[Consumer Products Guy]

Assume a PEG with average weight of 400.

Dissolve in DMF or pyridine, about 2 grams in 100 ml. Mix 250 ul with 500 ul BSTFA, cap and shake, inject. A traditional HP-5/DB-5 type column should do fine, ramp from maybe 100 C to 290 C, hold it there, split injection, FID. A high-temperature capillary would work fine as well.

[mimeo]

Hi Guy,

Thanks for your help, it is much appreciated. Is this the same proceedure for extracting PEG 400 from urine?

Take care

Rebecca

[Consumer Products Guy]

Personally, I would try to clean up the sample first (no pun intended) and also concentrate the PEG. I would run through mixed bed anion-cation ion-exchange resin in a column (by gravity), then rinse/wash/blow off the water/rinse solvent, then work up the residue. This will remove water and salts from your sample, concentrating the PEG.