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inlet tubing to and outlet tubing from the UV detector

http://www.chromforum.org/viewtopic.php?t=8386

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inlet tubing to and outlet tubing from the UV detector

Posted: Tue May 06, 2008 3:21 am
by moon704520
we have a prep-HPLC in our lab,but the OD of the tubing to the UV detector is 1/16,the rest part is 1/8OD, however,our working flowrate is 220ml/min,mobile phase 30%water:70%methanol(V:V) for the big column,so the back pressure is high as 7-8MPa,somebody recommend me the suitable tubing OD for the inlet tubing to the UVdetector?

Posted: Tue May 06, 2008 2:53 pm
by Chris0000
Hi

For flowrates greater 200 ml/min, I would use 1/8 tubing....

...but if you also use a small column you might have a flow dealy between detector and fraction collector at lower flow rates....

regards
Chris
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