autoclave/bioassays SOP
Posted: Mon May 05, 2008 8:15 pm
Hi all,
As a plant ecologist, I'm wondering how those in regulatory labs (or just more rigorous labs) would tackle the following problem.
I want to autoclave a set of compounds before using them as treatments in an experiment. My concern is that the autoclave might modify the structure of the compound (for example, one often adds antibiotics to media after autoclaving). How would one 'show' that autoclaving did not influence the structure of a compound? Is it sufficient to shoot the compound +/- autoclaving, and then use MANOVA on the peak areas in a pair of chromatograms? Would a either a product ion or neutral loss experiment be illustrative? That is, if I use lc as the front end and do a long gradient, is it enough to show that a particular daughter ion only emerges once (at the traditional RT)? I'm leaning towards the manova, but that approach consumes standard(s).
As a plant ecologist, I'm wondering how those in regulatory labs (or just more rigorous labs) would tackle the following problem.
I want to autoclave a set of compounds before using them as treatments in an experiment. My concern is that the autoclave might modify the structure of the compound (for example, one often adds antibiotics to media after autoclaving). How would one 'show' that autoclaving did not influence the structure of a compound? Is it sufficient to shoot the compound +/- autoclaving, and then use MANOVA on the peak areas in a pair of chromatograms? Would a either a product ion or neutral loss experiment be illustrative? That is, if I use lc as the front end and do a long gradient, is it enough to show that a particular daughter ion only emerges once (at the traditional RT)? I'm leaning towards the manova, but that approach consumes standard(s).