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Quantification of leucine in bacterial cells

http://www.chromforum.org/viewtopic.php?t=8383

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Quantification of leucine in bacterial cells

Posted: Mon May 05, 2008 10:37 am
by MariaRPB
Hello everybody!! :D

Do you know how can I make the quantification of leucine in bacterial cells? I want to remove bacterial cells from water samples, extract the leucine and make the quantification... Using HPLC how can I do this?

Plizzzzz!!!!! :lol:

Posted: Mon May 05, 2008 7:43 pm
by oscarBAL
Hello Maria;
You need to analize Total Leucine or free?
This is importat to define for the sample treatment.
what kind of water you have? is drinking waters; sea water waste water?
Certainly you can use HPLC for that you can use precolumn or postcolum derivatation.
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