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Atp, 1 drug, 3 peaks

http://www.chromforum.org/viewtopic.php?t=8378

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Atp, 1 drug, 3 peaks

Posted: Fri May 02, 2008 3:18 pm
by coquim
Hi everybody! :)

I´m almost sure but, however i would like your opinion...
I´m analyzing Atp and i´m having 3 peaks, and i suppose those peaks are AMP, ADP, and ATP.
I´m diluting Atp with water.
Probably ATP is losing 1 and 2 phosphates because its reactivity isn´it?
Thanks in advance!

Posted: Sun May 04, 2008 12:06 am
by XL
I believe you are right. Run the sample using both a highly aqueous compatible RP column and an anion-exchange column, and you will observe different elution order (or selectivity).

Posted: Wed May 07, 2008 2:31 am
by JSAS
you can purchase ATP, ADP, and AMP materials from Sigma and easily confirm the elution times
All times are UTC
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