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Seperation of PEG for GC analysis
Posted: Fri May 02, 2008 11:37 am
by mimeo
Hi there,
Can anyone tell me how to derivitize and seperate PEG 400 for GC analysis? Does the GC oven temperature have to be a high temp one?
Thanks in advance for any help or advice!
Rebecca
Posted: Mon May 05, 2008 6:31 pm
by Consumer Products Guy
Assume a PEG with average weight of 400.
Dissolve in DMF or pyridine, about 2 grams in 100 ml. Mix 250 ul with 500 ul BSTFA, cap and shake, inject. A traditional HP-5/DB-5 type column should do fine, ramp from maybe 100 C to 290 C, hold it there, split injection, FID. A high-temperature capillary would work fine as well.
Posted: Thu May 08, 2008 9:37 am
by mimeo
Hi Guy,
Thanks for your help, it is much appreciated. Is this the same proceedure for extracting PEG 400 from urine?
Take care
Rebecca
Posted: Thu May 08, 2008 6:52 pm
by Consumer Products Guy
Personally, I would try to clean up the sample first (no pun intended) and also concentrate the PEG. I would run through mixed bed anion-cation ion-exchange resin in a column (by gravity), then rinse/wash/blow off the water/rinse solvent, then work up the residue. This will remove water and salts from your sample, concentrating the PEG.