Column selection - thiamine pyrophosphate
Posted: Fri Mar 09, 2018 7:41 am
Hello,
For a project, i need to develop an LCMS method for thiamine pyrophosphate (TPP).
In our routine food analyses, we hydrolyze this compound to seperate and detect thiamine on a conventional C18 column. This is not an option in this project.
I'm looking for tips on the selection of an appropriate column type for TPP. I want to avoid adding (ion-pair) reagents to increase retention on a normal RPLC column because it has to be MS-friendly. I'm thinking in the direction of either a cyano type phase (RPLC) or HILIC. Anyone has experience with TPP, or can recommend a (U)HPLC column? I've searched for app notes / literature but I have difficulties finding a solution.
For a project, i need to develop an LCMS method for thiamine pyrophosphate (TPP).
In our routine food analyses, we hydrolyze this compound to seperate and detect thiamine on a conventional C18 column. This is not an option in this project.
I'm looking for tips on the selection of an appropriate column type for TPP. I want to avoid adding (ion-pair) reagents to increase retention on a normal RPLC column because it has to be MS-friendly. I'm thinking in the direction of either a cyano type phase (RPLC) or HILIC. Anyone has experience with TPP, or can recommend a (U)HPLC column? I've searched for app notes / literature but I have difficulties finding a solution.