Confirmation criteria for an LC MS-MS method

[hitelia]

Could someone give some ideas what confirmation criteria to use to confirm an analyte screened with an HPLC micromass quattro micro electrospray positve mode.

[MaryCarson]

Depends on which regulatory agency you need to satisfy.

[Kostas Petritis]

I would say that retention time and two MRM transitions that have a constant ratio under the same fragmentation conditons and independantly of analyte concentration should satisfy most of regulatory agencies (having said that I am not knowledgable on the exact requirements of these agencies).