Chromatography Forum

I am working with 2-Fluorophenyl acetic acid . I already seperated its isomers on HPLC . But the detection was not good in lower side [i.e. 0.02% or 0.05%] can any one suggest me that how i can analyse this material and its isomers on GC

Thans and regards

praveen

If I were to explore GC I would suggest an ASTEC capillary chiral GC column after esterifying the acid.

I would ask their technical service for suggestions.

techservice@sial.com

best wishes,

Rod

Thanks for reply.

I want to know from you that which type of chiral column is very suiytable for this kind of analysis.

Thans and regards

praveen

You wrote:

"I want to know from you that which type of chiral column is very suiytable for this kind of analysis.

Thans and regards

praveen"

OK, Proveen,

If given a choice between a world class expert in the techservice department, and me, a relative novice why would you choose to hear my opinion?

You must have internet service or how did you post here?

For your own benefit I again suggest you email techservice and get some expert advice. My advice would be buy and try and that might be expensive for you Experience would be the best choice and the expert in techservice has that quality.

Isomer separation for the fluorophenylacetic acid isomers in my experience is difficult and I do believe the best direction to pursue is a size exclusion GC column. Unfortuntately there are a lot of expensive choices and it is sometimes difficult to predict what will work and what will not. That is why the advice of an EXPERT is always the best choice. I hope you take that direction.

best wishes,

Rod