cysteine problem
Posted: Fri Apr 18, 2008 4:31 am
I purified a short peptide with 6 cysteine residues, one cysteine residue is between two alanine residues [A-C-A]. When it came to read this cysteine residue on the amino acid sequence machine, both cysteine and alanine were increased (alanine was higher). Is that a kind of misreading or......?
Also another cysteine residue at the end of this peptide wasn't detected, any clue......?!
Also, there are 2 tyrosine residues in this peptide, the concentration of one tyr residue was too little, does anybody know why?
I tested the specificity, Mr value, and gene ecoding for this peptide and everything was ok......
Thank you for your attention
Also another cysteine residue at the end of this peptide wasn't detected, any clue......?!
Also, there are 2 tyrosine residues in this peptide, the concentration of one tyr residue was too little, does anybody know why?
I tested the specificity, Mr value, and gene ecoding for this peptide and everything was ok......
Thank you for your attention
