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Large PEG separation

http://www.chromforum.org/viewtopic.php?t=8283

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Large PEG separation

Posted: Wed Apr 16, 2008 3:07 pm
by doddlebop
Hi all--
Can anyone recommend a method to separate large PEG's (1 million and higher). I used a standard C18 column for smaller ones and it worked wonderfully. I cannot separate these larger ones though, and have not seen a lot of publications on these larger ones.
TIA

Posted: Thu Apr 17, 2008 12:44 am
by Uwe Neue
The usual method for such problems is size-exclusion chromatography (SEC): Ultrahydrogel with water as the mobile phase. As a matter of fact, PEGs are used as standards in SEC.
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