Chromatography Forum

I don´t undesrtand very well this revelation, says that uses HPLC with a mass detector, and uses a KOH generator.

phase a->H2O per HPLC
column-> ion pac


Time %A mmolKOH
0 100 0,2
17 100 62,5
17,5 70 0,2
19,5 70 0,2

My questions are:
-What about he phase b?
-If it is 70 % of phase A, what is the rest?
-This system is an ionic-mass detecor or HPLC/MS?

This sounds like an Ion Chromatography system ( Dionex? ), and you should read the method details more carefully, as your method doesn't make sense to me. 100% A usually isn't both 0.2 mM and 62.5 mM KOH.

KOH is unlikely to be injected into most mass detectors, so there may be additional details that are specific to the instrument used.

If it's ion chromatography, then it's likely both the mobile phases are water with KOH, perhaps A is 0.2 mM KOH, and B is 62.5 mM KOH.

I'd suggest looking at the Column supplier's details, and contact them, or at least review relevant application notes at their website.

Bruce Hamilton

Thank very much for your response

It´s a dionex instrument.