columns for carbonyl analysis
Posted: Thu Feb 22, 2018 6:46 pm
by lvan
What is the best column to use for carbonyl analysis and what is your mobile phase? Trying to find a better column using a gradient mobile phase of acetonitrile, water and methanol.
Re: columns for carbonyl analysis
Posted: Thu Feb 22, 2018 7:20 pm
by mattmullaney
Hi Ivan,
Are you performing derivatization with DNPH? If so, try Supelco's RP-Amide, Agilent's Bonus RP, or Thermo's Acclaim Carbonyl columns. If you prefer SPPs, then try AMD's HALO 5 C18 (with some THF) or HALO-5 RP-Amide.