Analysis of drug(Glimepiride) in plasma sample
Posted: Mon Apr 14, 2008 6:42 am
Dear All
Really thank to all the peoples for replying this threat. One more thing i want to confirm if plasma interference eluting on the same Rt of compund and it is carry over just because of injector what type of rinsing solven we can use for the Injector. As i tried with 80% Acetinitrile in milli-Q water, 70% Methanol in milli-Q water. Mixture of ACN with Methanol in water. As i am analysing the Glimpiride and it is hydrobhobic compound. USP also suggested diluent as Acetonitrile:water (4:1).
Is there any chance that we can use Acetonitrile : Water(1:4) ratio if intereference is polar in nature and not getting clear of because of high organic content.
Kindly provide your valuable suggestion
PRASH
Really thank to all the peoples for replying this threat. One more thing i want to confirm if plasma interference eluting on the same Rt of compund and it is carry over just because of injector what type of rinsing solven we can use for the Injector. As i tried with 80% Acetinitrile in milli-Q water, 70% Methanol in milli-Q water. Mixture of ACN with Methanol in water. As i am analysing the Glimpiride and it is hydrobhobic compound. USP also suggested diluent as Acetonitrile:water (4:1).
Is there any chance that we can use Acetonitrile : Water(1:4) ratio if intereference is polar in nature and not getting clear of because of high organic content.
Kindly provide your valuable suggestion
PRASH