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Rolling, rolling, rolling
Posted: Fri Oct 01, 2004 1:47 pm
by mtnshawn
Having trouble figuring out the cause of my rolling baseline. The roller is very symmetrical and has a peak to valley time of approx. 10 minutes. Won't bore you w/ the details of my trouble shooting. Any thoughts?
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Posted: Fri Oct 01, 2004 2:11 pm
by Newman768
When the time between two injections correlates with the baseline rolling time you see a (very) late eluating peak.
Posted: Fri Oct 01, 2004 3:55 pm
by mtnshawn
the rolling baseline occurs during equilibration as well as during runs.
Posted: Fri Oct 01, 2004 10:24 pm
by Uwe Neue
How about temperature fluctuations? Air conditioning going off and on? Column heater off/on?
Posted: Mon Oct 04, 2004 2:28 pm
by Newman768
If the problem is caused by a late eluating peak, you get rid off the problem by flushing the column with high % to 100% organic solvent.
The next equilibrating with your normal mobile phase should show a smooth baseline.
Using a solvent with 10 to 20% less water show such a compound coming from the sample(s) within normal run time.