PITC heptane extraction procedure?
Posted: Fri Oct 01, 2004 1:34 pm
Hello,
I'm working on a simple method to quantitate free amino acids using PITC. However, my lab is currently unequiped with a vacum for PITC extraction from the sample so I'm planning on using Heptane extraction. Does anyone know how to do a Heptane extraction? If I mix the heptane with my sample consisting of EtOH:H2O:TEA:PITC (7:1:1:1) how will the heptane seperate the PITC from the derivatized amino acid? Any help will be much appreciated.
PS One last question, would Hexane work in place of Heptane?
I'm working on a simple method to quantitate free amino acids using PITC. However, my lab is currently unequiped with a vacum for PITC extraction from the sample so I'm planning on using Heptane extraction. Does anyone know how to do a Heptane extraction? If I mix the heptane with my sample consisting of EtOH:H2O:TEA:PITC (7:1:1:1) how will the heptane seperate the PITC from the derivatized amino acid? Any help will be much appreciated.
PS One last question, would Hexane work in place of Heptane?