anion exchange method for cu-edta chelate?
Posted: Sun Apr 06, 2008 11:50 am
by alicee!
hii.. does anyone have an anion exchange method for cu-edta chelate determination and quantitation?
i have a weak anion exchange column to use.. any methods for that? or anything else?
Posted: Sun Apr 06, 2008 1:12 pm
by SIELC_Tech
Posted: Sun Apr 06, 2008 3:19 pm
by alicee!
i;d try this.. why H2SO4 by the way? only pH? or to avoid any other acid's use? why so?
why methanol for ion exhange? i would be using only anion exchnge column.. without an rp base.. so i need not use methanol right? thankss!!
Posted: Sun Apr 06, 2008 4:15 pm
by SIELC_Tech
you need ions in the mobile phase to facilitate anion-exchange and elute EDTA from the column. Strong acid will help you obtaining better peak shape. You can use any acid or buffer but sulfuric acid and TFA will work the best. I would stay away from methanol for this particular application.
