I am new to HPLC... Pl. help me!!!

[sechau1]

Hi, I am a newbie to HPLC. I have to use SEC-HPLC for analytical purpose to see %monomer, %aggregate etc from a purified Ab (M.Wt: 150KDa). We have an old Waters 2695 in lab. Could you please suggest what column (if possible brand/model) that I should use? Also, do I need several columns?
Thanks in advance.
A lost Grad student

[Uwe Neue]

Either use a Protein-Pak 300 7.5 x 300 or a BioSuite SEC 250 7.8 x 300 column (both Waters) at a flow rate of 1 mL/min, phosphate buffer pH 7, about 25 mM plus 100 mM NaCl. You can separate the monomer from the dimer on a single column.