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how to remove residual TBAH - ion-pair agent from column..?

Posted: Sat Apr 05, 2008 4:24 pm
by alicee!
hi all.. how do you guys remove the residual TBAH - ion-pair agent bound to rp columns.. when doing an ion-pair chromatography?

Posted: Sat Apr 05, 2008 10:21 pm
by tom jupille
Generally speaking, wash with a mildly acidic buffer + organic solvent. There is no guarantee that you will get it all off.