Expert suggestions on establishment of Mass Spec Facility

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

7 posts Page 1 of 1
We are revamping our existing mass spec facility. We currently have the following instruments.

1. Impact HD Q-TOF (Bruker)
2. Single Quad GC-MS (Agilent)

We are a central facility where we will be doing all sorts of samples. Proteomics, metabolomics, different and various biological applications, like modification study, structure study, small molecular structure prediction, etc. In a word whatever is possible.

My request to all the experts here is please suggest which instruments we should procure and for what applications.

Looking forward to your response.
Hi,

There are tons of options out there but I would definitely start with some kind of triple quad... AB Sciex 4500, 5500, or 6500.

The folks at McKinley Scientific can provide impressively flexible leasing and financing options on both new and preowned equipment FYI.
All depends on what you're analyzing, and what your goal is.

Agilent has a new 7250 GC QTOF that is very nice. LC QTOF is great too, everything after the quadrupole is basically the same.. just the front end differs for GC/LC. That's what I'd go with if I had money to spend and need a really powerful MS.

But it depends what you're trying to do. Do you need accurate mass? If so, then yeah you need to go with QTOF. Do you need selectivity in a complex sample matrix and lots of analytes? Go tandem quad. Need good quality scan data for library matching and identification and want multiple instruments for high throughput? Single quad...
Your question is too broad to answer. If you want to invest in the most appropriate equipment, then hire a consultant with many years of practical instrument experience to assist you map out your goals, applications, budget and site prep needs. Front-end work to get some help and training is well worth the investment before you spend big money.
aldehyde wrote:
All depends on what you're analyzing, and what your goal is.

Agilent has a new 7250 GC QTOF that is very nice. LC QTOF is great too, everything after the quadrupole is basically the same.. just the front end differs for GC/LC. That's what I'd go with if I had money to spend and need a really powerful MS.

But it depends what you're trying to do. Do you need accurate mass? If so, then yeah you need to go with QTOF. Do you need selectivity in a complex sample matrix and lots of analytes? Go tandem quad. Need good quality scan data for library matching and identification and want multiple instruments for high throughput? Single quad...


If you need both triple quad MSMS type work and single quad MS work, then choose a good triple which can operate in both modes. We did this with the Agilent 7000, which can work for doing pest residues in food matrix with QQQ and also run drinking water samples in single quad scan or SIM. That way the instrument doesn't sit idle when there are no food samples to run.
The past is there to guide us into the future, not to dwell in.
James_Ball wrote:

If you need both triple quad MSMS type work and single quad MS work, then choose a good triple which can operate in both modes. We did this with the Agilent 7000, which can work for doing pest residues in food matrix with QQQ and also run drinking water samples in single quad scan or SIM. That way the instrument doesn't sit idle when there are no food samples to run.


Hope it is running well for you. 7000s are very nice instruments.

They really shine for MRMs, but there are a lot of very useful acquisition modes.
Guys, thank you for your sincere responses.
7 posts Page 1 of 1

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