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CuEDTA chelate..pKa value?can i use pHrange 4-2 fr anionxchg

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CuEDTA chelate..pKa value?can i use pHrange 4-2 fr anionxchg

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alicee!
hi all.. i needed confirm the Cu-EDTA chelate pKa value?
what i vaguely read was it would be cuedta2- at pH 3 while near 2.7 it would be neutral? is that right? hence, can i use pH range from 4 for binding and up to 2 for elution.. on an anion exchange column.. i mean a DEAE column?
i cudnt find any papers on anion exchange for EDTA quantitation.. anybody? thanks!!
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rhaefe
http://www.hamiltoncomp.com/cgi-shl/pro ... pplist2134
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Robert Haefele

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Bryan Evans
Below is a reverse phase method (if you require that):
http://www.silvertonesciences.com/files/TI261E.pdf
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