Chromatography Forum

hello everybody,

I am working with my best friend agilent 1200 hplc:)

I have not had any problems with peak purity until now;
I was doing analysis of methyl and aethyl paraben, then i was injecting benzyl alcohol; unfortunately, on alkohol chromatogram i cannot eliminate peaks (small, but they are still at chromatogram) coming from parabens ; I was using option of injection with washing using .... vial; I know that there is an option of writing short program inside the method; it concerns thing like draw ...... from vial, eject from ..... vial and so on but i do not know how to write it properly (the easiest way)

can somebody write me these view lines of program

(i did :
1. draw ..... from vial 100
2.eject .... to vial 99
3.draw ...... from sample, which was my target sequence vial
4.inject

but it did not work very well;
I mean the peak was assymetrical, and sometimes doubled; I want to emphasized that if I was using standart procedure of injection, without the program, peak was symmetrical and single

Click the needle wash option in the Autosampler section. Put a wash vial in the location chosen.