Chromatography Forum

I would like to program two sequence consecutive on the chemstation form Agilent .
pleas Help

Isn't possible to add extra rows into first sequence?
I think that ChemStation doesn't support sequence chaining.

Edit:
Maybe I don't understand your problem correctly. If you want to prepare another sequence on "running" instrument, reprocess copy of Chemstation (View-> Reprocessing copy), switch to View->Method and Run Control and create your second sequence (then save).

There is an option in the ChemStation called "Hypersequence." However, it can only be used with the well-plate autosampler. This option is not available with the standard autosampler.

But you can program multiple methods and samples into one sequence. And you can add lines to the sequence during the run.

Thank's for all you Help

You can use the ChemStation Scheduler as follows. You will first need to know how long the first sequence will run. Assume that it takes 4 hours to run the first sequence and you wish to start it at 17.00.
1. Enter the date and for the time enter 17.00.

2. For the command type in RunSequence.

3. For the mode leave as "Do Once"

4. On the second line enter the date and 21.00 for the time ( 4 hours plus in this exanple.

5. For the command enter LoadSequence "C\Chem32\1\Sequence\","Name.S". The first entry should be the path where you sequence is stored and the second entry the name of the sequence.

6. Leave the mode as "Do Once"

7. On the third line, enter the date and the time as entered in 4 above plus 5 minutes.

8. For the command enter RunSequnce

The scheduler will then start the first sequnce at 17.00. Four hours later it will load the second sequence and start that 5 minutes later.

Gasman

Clever.

I'm not sure the poster is looking for anything that sophisticated. Just enter into the Sequence Table the first row of the second sequence after the last line of the first sequence, different Method.M is no issue. For HPLC Chemstation, you may want to program in a 15 minute Wait Time Before Starting a Sequence (in Sequence Parameters) so the HPLC can equilibrate after loading the second Method.M. We do this all the time, even using an HPLC column switching valve (1100) to use a second column for the second method.M.

There is a subdirectory on the ChemStation CD ROM that is \UCL (for user contributed Library), in there is \LC_UCL\Macros. Look in the index, there is a macro for chaining sequences. When it finishes the first sequence, the chain sequence macro will look in its folder to see if there is another, and load and run any sequences. We wrote that a few years ago.