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How can I calculate de Void Volume of a strong anionic excha

http://www.chromforum.org/viewtopic.php?t=80604

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How can I calculate de Void Volume of a strong anionic excha

Posted: Fri Jan 12, 2018 3:16 pm
by apotolicchio
Hi, I read examples of particles used for calculating the Vo in gel filtration (dextran blue) or Reversed Phase (urea), but I'm having trouble finding an unretained equivalent for IEX chromatography.

How can I calculate de Void Volume of a strong anionic exchange column (Q FF)? What unretained particle can I easily use for this purpose?
Note: the column I'll be using is a bench column, manual, not HPLC this time.

Thanks!

Re: How can I calculate de Void Volume of a strong anionic excha

Posted: Fri Jan 12, 2018 4:55 pm
by Multidimensional
I think you posted this same question on another website. You question was answered too.

Re: How can I calculate de Void Volume of a strong anionic excha

Posted: Fri Jan 12, 2018 5:55 pm
by mattmullaney
Hi apotolicchio,

I found this discussion in the archives:

http://www.lcresources.com/discus/messa ... 20041222pm

and you can try the suggested mix on pg. 14 of this reference:

https://www.auburn.edu/~duinedu/manuals/QSepharose.pdf

Best Wishes!
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