quntitation by gc-ms??
Posted: Thu Feb 28, 2008 3:32 pm
If I have a peak in TIC for morphine dissolved in methanol.
How can I quantitate it in mg%??
How can I quantitate it in mg%??
Simple.... with calibration
Prepare a sample of known concentration in the same matrix, e.g.10mg/mL in methanol. Run your unknown sample and the known sample and compare peak areas.
unknown peak area / known peak area x known concentration = unknown concentration in solution.
That's obviously a single point calibration, if you want more accuracy, run a calibration curve and create a linear regression equation.
If you want a weight% of a solid sample (e.g. drug) take the dilution factor into account when back-calculating.