Advertisement

Area % method

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
For salt stability testing experiment my boss has asked to use area % method. Can somebody please explain me that

Thanks a lot in advance

Add up the total area for all the peaks, then calculate the % of that total represented for each peak of interest. The assumption is that the response factors for all the peaks are the same (a very risky assumption, but one that is often made in the absence of standards for all the degradants) so that the area % is the same as the mass %.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374

Peaks resulting from an injection of a blank solution should not be included in the calculation.
3 posts Page 1 of 1

Who is online

In total there are 25 users online :: 4 registered, 0 hidden and 21 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Ahrefs [Bot], Google [Bot], Google Adsense [Bot], Semrush [Bot] and 21 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry