HPLC-FD surrogate standard for PAH analyses
Posted: Mon Sep 27, 2004 12:44 pm
Hi everybody in this my first message 
I intend to use surrogate/internal standards for PAHs analyses using HPLC with fluorescence detection. I don't wan't to use deuterated compounds (by the coelution problems) so I have tried the compound offered by Dr. Ehrenstorfer catalog: 1,3,5-triphenylbezene.
After the injection of 10 ppm and 1 ppm solutions I have two problems:
1) Poor sensitivity
2) Chromatograms with two peaks: one before naphthalene retention time (~10 min), and the other before benz[a]antracene RT (~23 min)
I think that the "real" peak must be the later one
I need to do more tests, and I would be grateful for some help:
Somebody else use this surrogate standard?
Any coments about the sensitivity (excitation/emission wavelenghts), and RT?
It would be better the use of another compound (may be p-terphenyl)?
Thanks a lot!
I intend to use surrogate/internal standards for PAHs analyses using HPLC with fluorescence detection. I don't wan't to use deuterated compounds (by the coelution problems) so I have tried the compound offered by Dr. Ehrenstorfer catalog: 1,3,5-triphenylbezene.
After the injection of 10 ppm and 1 ppm solutions I have two problems:
1) Poor sensitivity
2) Chromatograms with two peaks: one before naphthalene retention time (~10 min), and the other before benz[a]antracene RT (~23 min)
I think that the "real" peak must be the later one
I need to do more tests, and I would be grateful for some help:
Somebody else use this surrogate standard?
Any coments about the sensitivity (excitation/emission wavelenghts), and RT?
It would be better the use of another compound (may be p-terphenyl)?
Thanks a lot!