HPLC method for Zoledronic acid

[N.V.V.S.S. RAMAN]

Hai,

Can any body suggest an HPLC method for Zoledronic acid which is a non chromophore compound.

Raman

[AA]

Chromatographia
Publisher: Friedrich Vieweg & Sohn-Verlag GmbH & Co.KG
ISSN: 0009-5893 (Paper) 1612-1112 (Online)
DOI: 10.1365/s10337-004-0384-8
Issue: Online First

Analysis of Zoledronic Acid and Its Related Substances by Ion-Pair RP-LC
Y. Jiang1 , X. Q. Zhang1 and Z. R. Xu1

(1) Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road of Shijiazhuang City, China

Received: 5 January 2004 Revised: 4 June 2004 Accepted: 14 June 2004 Published online: 18 August 2004

[Einar Ponten]

This is highly hydrophilic biphosphonate compound.

I suggest that you try a ZIC®-HILIC column, using 80% acetonitrile/20% ammonium acetate buffer. Our experience with compounds like ATP are a promising indication for this application as well.

[SIELC_Tech]

Your compound will retain similar to cidofovir (polar hydrophilic coompound with phosphate group). You can compare it to regular C18 column.

http://allsep.com/makeChr.php?chr=Chr_068

[kalyanaraman]

Being a hydrophilic bisphosphonate, retention in a reverse phase column will be very difficult. Also peaks may tail because of the Imidazole moiety in the compound. Also the compound will respond only in lower UV region. To solve these issues a quarternary ammonium ion pair (use phosphate or sulphate salts for UV cutoff) mobile phase using a 100% aqeous compatible reverse phase column will help you.