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hi all.. how do you guys remove the residual TBAH - ion-pair agent bound to rp columns.. when doing an ion-pair chromatography?
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how to remove residual TBAH - ion-pair agent from column..?
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
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- tom jupille
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Generally speaking, wash with a mildly acidic buffer + organic solvent. There is no guarantee that you will get it all off.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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