Chromatography Forum

I was looking through some literature and noticed that TOSOH offers a column which is essentially a polymeric version of an amino column, that they advocate for normal phase.

I thought this was interesting. I have never been a big fan of polymeric columns, but it seems like they might make more sense for normal phase: because the back pressure is much lower, and because the polymeric backbone won't contribute to excessive retention like it can sometimes when doing reversed phase HPLC with polymer columns.

This also caught my attention because amino columns are uniquely useful for certain normal phase applications, but can be very reactive. These polymeric columns are probably more stable.

Anyone ever tried this? Any comments?

Traditional silica-based NH2 phases are unstable when exposed
to aqueous eluent - resulting in loss in retention / increase in detector
noise (e.g. ELSD). Polymeric NH2 phases are more stable - but suffer
from poor efficiency.

Unison UK-Amino (silica-based) is durable under aqueous eluent - we have the data
to support this. If you let us know what type of compounds you are
interested in - I can post some related chromatograms. Thanks