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Integration methods
Posted: Mon Feb 11, 2008 9:36 pm
by lmatthe
Does anyone have a good reference that describes "accepted" methods of peak integration? I have seen that ASTM has a standard on peak integration, but I do not have access to it.
Any help would be greatly appreciated!
Posted: Tue Feb 12, 2008 2:15 pm
by danko
Hi Laura,
It’s an interesting topic, but difficult to discus without a concrete example. Do you have data showing the issue? Or is it just a general discussion you’d like to start? The latter may be too large to come around in this forum. But if you post a chromatogram, demonstrating the different options you have, maybe it’ll facilitate the discussion.
I personally always insist on a blank injection/run, guiding the user through the baseline. So when the blank and the sample are overlaid, the baselines should align perfectly.
I’ve seen terrible examples of crawling baseline (in cases of inadequate separation) in order to obtain “valley to valleyâ€
Posted: Tue Feb 12, 2008 2:39 pm
by DR
Rules of thumb:
You want at least 20 points over the course of your smaller peaks
Consistency is good
Even the very best CDS can't fix bad chromatography
Beyond that, it's pretty much a function of figuring out what parameters and/or events will yield reasonable, consistent integration throughout a given data set.
Posted: Wed Feb 13, 2008 6:33 am
by tom jupille
If you want to get into details, the "standard" reference is Dyson's book on Chromatographic Integration Techniques:
http://tinyurl.com/yskt76
DR actually gave you the "bottom line". The most important part of which is
Even the very best CDS can't fix bad chromatography