Calibration of One analyte with multiple peaks (Chromeleon)

[Fat Jonny]

I have a compound made up of 6 oligomers which I am running by a simple reverse phase HPLC using an Acetonitrile/water gradient.

Our approach will be to simply sum the areas of the 6 oligomer peaks and plot against concentration to create a calibration curve. We want to use all 6 areas to maximise the LOD.

I'm trying to figure out how to do this using the Dionex Chromeleon software. Does anybody have any idea how to edit the integration/quantitation method file to do this? I've only ever worked on one peak = one analyte before.

Thanks in advance!

[unmgvar]

Hi Fat Jonny

if i understood correctly,

you have 6 peaks in your chromatograms both in the standards and in the samples and you want to calcualte them as a sum.

there several "ways" of approach to my knowledge, depending on what you will exactely need or how you want to calculate
the first approch is to "group" the peaks.
go to the QNT in the peak table section. there you have the column "group"
input the same value in the field for your peaks. for example, type apples for all of them.
for your standard amount you need to choose one of the peaks and insert the value for him only, put 0.0 for all others (i am assuming that the standard is also made of several oligomers as well).

this should do the trick

[Fat Jonny]

Thanks working perfectly, I thought it would have something to do with the "group" option. I also had to change the "amount" column in the report format to the "group amount" option.

Works perfectly!